TitleA novel and rapid method for synthesizing positive controls and standards for quantitative PCR.
Publication TypeJournal Article
Year of Publication2008
AuthorsDavid MM, Sapkota AR, Simonet P, Vogel TM
JournalJ Microbiol Methods
Volume73
Issue1
Pagination73-7
Date Published2008 Apr
ISSN0167-7012
KeywordsCalibration, DNA Primers, DNA, Bacterial, Nucleic Acid Hybridization, Polymerase Chain Reaction, Reference Standards, Sensitivity and Specificity
Abstract

We developed and tested a method to produce DNA standards and controls for quantitative PCR by designing and performing partial hybridization of long oligonucleotides before double stranded DNA fragments were synthesized and subsequently amplified by conventional PCR. This approach does not require any natural DNA template. Applications include the production of standards, which cannot be easily produced from DNA extracted from bacteria or plants.

DOI10.1016/j.mimet.2008.01.001
Alternate JournalJ. Microbiol. Methods
PubMed ID18313777