TitleModeling of phenoxy acid herbicide mineralization and growth of microbial degraders in 15 soils monitored by quantitative real-time PCR of the functional tfdA gene.
Publication TypeJournal Article
Year of Publication2012
AuthorsBælum J, Prestat E, David MM, Strobel BW, Jacobsen CS
JournalAppl Environ Microbiol
Volume78
Issue15
Pagination5305-12
Date Published2012 Aug
ISSN1098-5336
Keywords2,4-Dichlorophenoxyacetic Acid, 2-Methyl-4-chlorophenoxyacetic Acid, Dioxygenases, DNA Primers, Genes, Bacterial, Herbicides, Kinetics, Minerals, Models, Biological, Real-Time Polymerase Chain Reaction, Soil Microbiology
Abstract

Mineralization potentials, rates, and kinetics of the three phenoxy acid (PA) herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D), 4-chloro-2-methylphenoxyacetic acid (MCPA), and 2-(4-chloro-2-methylphenoxy)propanoic acid (MCPP), were investigated and compared in 15 soils collected from five continents. The mineralization patterns were fitted by zero/linear or exponential growth forms of the three-half-order models and by logarithmic (log), first-order, or zero-order kinetic models. Prior and subsequent to the mineralization event, tfdA genes were quantified using real-time PCR to estimate the genetic potential for degrading PA in the soils. In 25 of the 45 mineralization scenarios, ∼60% mineralization was observed within 118 days. Elevated concentrations of tfdA in the range 1 × 10(5) to 5 × 10(7) gene copies g(-1) of soil were observed in soils where mineralization could be described by using growth-linked kinetic models. A clear trend was observed that the mineralization rates of the three PAs occurred in the order 2,4-D > MCPA > MCPP, and a correlation was observed between rapid mineralization and soils exposed to PA previously. Finally, for 2,4-D mineralization, all seven mineralization patterns which were best fitted by the exponential model yielded a higher tfdA gene potential after mineralization had occurred than the three mineralization patterns best fitted by the Lin model.

DOI10.1128/AEM.00990-12
Alternate JournalAppl. Environ. Microbiol.
PubMed ID22635998
PubMed Central IDPMC3416398