TitleEvaluation of functional gene enrichment in a soil metagenomic clone library.
Publication TypeJournal Article
Year of Publication2009
AuthorsDemanèche S, David MM, Navarro E, Simonet P, Vogel TM
JournalJ Microbiol Methods
Volume76
Issue1
Pagination105-7
Date Published2009 Jan
ISSN0167-7012
KeywordsBacteria, Bacterial Proteins, Gene Library, Genome, Bacterial, Nucleic Acid Hybridization, Oligonucleotide Probes, Soil Microbiology
Abstract

We evaluated the use of mixed oligonucleotide probes hybridized to metagenomic clones spotted on high density membranes. The pooled probes included oligonucleotides designed for genes associated with denitrification, antibiotic resistance, and dehalogenation among others. Pyrosequence comparison between the clones and the original DNA demonstrated the utility of clone screening with pooled probes.

DOI10.1016/j.mimet.2008.09.009
Alternate JournalJ. Microbiol. Methods
PubMed ID18922230