Title | Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Authors | Demanèche S, Philippot L, David MM, Navarro E, Vogel TM, Simonet P |
Journal | Appl Environ Microbiol |
Volume | 75 |
Issue | 2 |
Pagination | 534-7 |
Date Published | 2009 Jan |
ISSN | 1098-5336 |
Keywords | Bacteria, Bacterial Proteins, DNA, Bacterial, Gene Order, Metabolic Networks and Pathways, Molecular Sequence Data, Multigene Family, Nitrogen, Operon, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Soil Microbiology |
Abstract | We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters. |
DOI | 10.1128/AEM.01706-08 |
Alternate Journal | Appl. Environ. Microbiol. |
PubMed ID | 19011059 |
PubMed Central ID | PMC2620714 |