TitleCharacterization of denitrification gene clusters of soil bacteria via a metagenomic approach.
Publication TypeJournal Article
Year of Publication2009
AuthorsDemanèche S, Philippot L, David MM, Navarro E, Vogel TM, Simonet P
JournalAppl Environ Microbiol
Volume75
Issue2
Pagination534-7
Date Published2009 Jan
ISSN1098-5336
KeywordsBacteria, Bacterial Proteins, DNA, Bacterial, Gene Order, Metabolic Networks and Pathways, Molecular Sequence Data, Multigene Family, Nitrogen, Operon, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Soil Microbiology
Abstract

We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters.

DOI10.1128/AEM.01706-08
Alternate JournalAppl. Environ. Microbiol.
PubMed ID19011059
PubMed Central IDPMC2620714